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Registros recuperados: 148 | |
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Abolhassani,Mohsen. |
Borage (Echium amoenum) is a large annual plant of the Boraginaceae family, which grows in most of Europe and in northern Iran. The borage flower is used as a medicinal herb in France and other countries. Iranian borage is used in traditional medicine for infectious diseases, flu and as an anti-febrile. We tested the aqueous extract of borage dried flowers in vitro for its antibacterial activity. The extract showed concentration-dependent antibacterial activity against Staphylococcus aureus 8327. This activity was heat resistant, but the activity of freeze-dried extract gradually diminished during a 90-day period. The traditional use of Iranian borage flowers for infectious diseases and for controlling fever appears to be justified. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Borago; Echium amoenum; Antibacterial activity; Staphylococcus aureus. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500008 |
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Adaleti,Riza; Nakipoglu,Yasar; Ceran,Nurgul; Tasdemir,Cihan; Kaya,Fatma; Tasdemir,Semiha. |
The incidence of drug-resistant pathogens differs greatly between countries according to differences in the usage of antibiotics. The purpose of this study was to investigate the phenotypic resistance of 321 methicillin resistance Staphylococcus aureus (MRSA) and 195 methicillin susceptible S. aureus (MSSA) in a total of 516 S. aureus strains to macrolide, lincosamide, streptogramin B (MLS B), ketolid, and linezolid. Disk diffusion method was applied to determine MLS B phenotype and susceptibility to different antibiotic agents. It was found that 54.6% of the isolates were resistant to erythromycin (ERSA), 48% to clindamycin, 55% to azithromycin, 58.7% to spiramycin, 34.7% to telithromycin, and 0.4% to quinupristin-dalfopristin, respectively. No strain... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Macrolide; Lincosamide; Streptogramin B; Ketolid; Linezolid; MLS B. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100003 |
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Adesida,Solayide A.; Abioye,Olusegun A.; Bamiro,Babajide S.; Brai,Bartholomew I.C.; Smith,Stella I.; Amisu,Kehinde O.; Ehichioya,Deborah U.; Ogunsola,Folasade T.; Coker,Akitoye O.. |
Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; PFGE; Nasal isolates; Carriage rate. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016 |
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Amaral,L.A.; Rossi Júnior,O.D.; Nader Filho,A.; Ferreira,F.L.A.; Barros,L.S.S.. |
Com o objetivo de verificar o papel da água utilizada durante a produção do leite como via de transmissão de Staphylococcus sp., fez-se a contagem de Staphylococcus coagulase negativa e Staphylococcus aureus nas amostras de água das fontes, dos reservatórios e dos estábulos de 30 propriedades leiteiras situadas na região Nordeste do Estado de São Paulo. As maiores ocorrências de isolamentos (10,0 e 16,6%) e os maiores valores médios (4,3×10(4) e 2,5×10(4)) de contagens desses microrganismos foram encontrados nas amostras de água dos estábulos utilizada na obtenção de leite. Estes resultados são importantes pois evidenciam a possibilidade de contaminação do leite ou dos animais por cepas patogênicas. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Água; Staphylococcus sp.; Staphylococcus aureus; Propriedade leiteira. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000500017 |
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Assumpção,E.G.; Piccoli-Valle,R.H.; Hirsch,D.; Abreu,L.R.. |
Com o objetivo de identificar as principais fontes de contaminação por Staphylococcus aureus e Staphylococcus produtores de coagulase (SC+), avaliou-se o processo de fabricação de queijo prato em um laticínio de Lavras, MG, durante os meses de outubro de 2000 a abril de 2001. As análises microbiológicas foram feitas no leite cru, no leite pasteurizado resfriado, nas mãos e antebraços dos funcionários, na salmoura, na água de imersão das fôrmas e no queijo embalado. Contagens elevadas de SC+ e de S. aureus (4x10³ a 4,8x10(6) UFC/ml e 4x10³ a 3,3x10(5) UFC/ml, respectivamente) foram encontradas em quatro avaliações no leite cru. Após a pasteurização, as contagens foram reduzidas a <1 UFC/ml. Em três das cinco avaliações, o queijo prato apresentou... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Queijo prato; Staphylococcus aureus; Condição higienico-sanitária. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300019 |
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Baraboutis,Ioannis G; Tsagalou,Eleftheria P; Papakonstantinou,Ilias; Marangos,Markos N; Gogos,Charalambos; Skoutelis,Athanasios T; Bassaris,Haralambos; Johnson,Stuart. |
OBJECTIVES: Both total antimicrobial use and specific antimicrobials have been implicated as risk factors for healthcare-associated methicillin-resistant Staphylococcus aureus (HCA-MRSA) infection. The aims of this study were: (I) to explore predictors of a new HCA-MRSA infection in comparison with a new healthcare-associated methicillin-sensitive Staphylococcus aureus (HCA-MSSA); (II) to thoroughly assess the role of recent antibiotic use qualitatively and quantitatively. METHODS: The time-period for our study was from October 1997 through September 2001. Through applying strict criteria, we identified two groups of inpatients, one with a new HCA-MRSA infection and one with a new HCA-MSSA infection. We recorded demographic, clinical and antibiotic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Staphylococcus aureus; Antibacterial agents; Fluoroquinolones. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500003 |
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Bazzaz,Bibi Sedigheh Fazly; Memariani,Zahra; Khashiarmanesh,Zahra; Iranshahi,Mehrdad; Naderinasab,Mahbobeh. |
Galbanic acid, a sesquiterpene coumarin from Ferula szowitsiana roots, was investigated for its potentiating effect on the antimicrobial activity of antibiotics as well as ethidium bromide, in 6 multidrug resistance (MDR) clinical isolates of Staphylococcus aureus. Galbanic acid had inhibitory effect on none of the isolated bacteria tested (up to 800 µg /ml). The MIC range of ciprofloxacin, tetracycline and ethidium bromide, against all tested S. aureus were 10-80, 10-80 and 4-16 µg/ml, respectively. These were reduced to ≤2.5-5, 2.5-5 and 0.5-2 µg/ml in the presence of galbanic acid (300 µg /ml) or verapamil (100 µg /ml). The rate of ethidium bromide (2 µg /ml) accumulation in clinical isolates was enhanced with galbanic acid (300 µg /ml). There is also a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Efflux pump inhibitor; Ferula szowitsiana; Galbanic acid; Staphylococcus aureus. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300006 |
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Beltrame,C. O.; Côrtes,M. F.; Bandeira,P. T.; Figueiredo,A. M. S.. |
Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA preparation; MRSA; Biofilm; Sessile cells; Staphylococcus aureus. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201071 |
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Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema. |
Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019 |
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Berberian,Griselda; Firpo,Verónica; Soto,Adriana; Lopez Mañan,Julio; Torroija,Cecilia; Castro,Graciela; Polanuer,Pablo; Espinola,Camilo; Piñeiro,José Luis; Rosanova,María Teresa. |
OBJECTIVES: The aim of this study was to identify the clinical, radiological, and bacteriological features, risk factors, and outcome of neonates with bone and joint infections. STUDY DESIGN: Observational, retrospective, and analytical study of 77 patients less than 2 months of age, admitted to a tertiary neonatal intensive care unit (NICU) with the diagnosis of bone or joint infection, based on clinical, radiological, and microbiological criteria. RESULTS: Seventy-seven patients with 99 acute osteoarthritis foci in a 16 year period were included in the study. Risk factors for infection could be identified in 69% of the patients. The hip was the most frequent. Staphylococcus aureus was the main isolated microorganism. Twenty-nine infants (38%) had... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Osteoarthritis; Neonate; Staphylococcus aureus. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400018 |
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Bonesso,Mariana Fávero; Marques,Silvio Alencar; Camargo,Carlos Henrique; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da. |
The aim of this study was to determine the prevalence of Staphylococcus aureus and risk factors for the acquisition of MRSA (Methicillin Resistant Staphylococcus aureus) as the main cause of skin and soft tissue infections. S. aureus were characterized for the presence of PVL, TSST-1 and mecA genes. SCCmec typing was carried out in mecA positive strains and PFGE was performed only in these strains. During the study period, 127 outpatients attending a dermatology clinical the Botucatu Medical School, a regional tertiary hospital in Botucatu, Sao Paulo, Brazil, were diagnosed with active skin infections. A total 66 (56.9%) S. aureus strains were isolated. The methicillin resistance gene mecA was detected in seven (10.6%) S. aureus strains. The SCCmec types... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; Resistance; Skin infections; Virulence; Epidemiology. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400034 |
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Brites,Carlos; Silva,Nanci; Sampaio- Sá,Márcia. |
Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have become an increasing problem in Brazilian hospitals within the last years. In Bahia, there is scarce information on the epidemiological characteristics of MRSA infections and their determinants. The objective of this study was to evaluate the temporal evolution of MRSA infections in a private, tertiary hospital, in Salvador, the capital of the state of Bahia. We reviewed the microbiological records of bacterial isolates from the Hospital Espanhol, a 300 bed, general hospital, in Salvador. We analyzed the frequency of positive cultures for S. aureus during the last nine years, the prevalence of MRSA, and the spatial distribution of the isolates in the hospital. We also evaluated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus aureus; MRSA; Bacterial resistance. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400003 |
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Brito,M.A.V.P.; Brito,J.R.F.; Cordeiro,F.M.; Costa,W.A.; Fortes,T.O.. |
Duzentos e dezoito amostras de Staphylococcus aureus, isoladas de infecção intramamária de vacas de 44 rebanhos leiteiros, foram classificadas em biótipos de acordo com os testes de produção de estafiloquinase (K), beta-hemolisina (beta ), coagulação do plasma bovino (Pl) e crescimento na presença de cristal violeta (CV). As amostras foram distribuídas em 10 biótipos e 63 delas foram classificadas nas ecovariedades bovina (35), ovina (17), aviária (10) e humana (1) e 155 não apresentaram características específicas de hospedeiro. Estas últimas podem ser isoladas de homem, cabra, coelho, suíno, alimentos e de mastite bovina. O biótipo 1, encontrado com maior freqüência (37,2%), apresentou o padrão K (-), beta (+), Pl (-) e CV (azul). Em sete rebanhos nos... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Mastite; Staphylococcus aureus; Biótipo. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000500001 |
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Brito,M.A.V.P.; Brito,J.R.F.; Silva,M.A.S.; Carmo,R.A.. |
Determinou-se a concentração mínima inibitória (CMI) de ampicilina, cefalotina, eritromicina, gentamicina, neomicina, norfloxacina, oxacilina, penicilina G, tetraciclina e tilosina para 112 amostras de Staphylococcus aureus isoladas de infecções intramamárias bovina, em 33 rebanhos leiteiros da Zona da Mata do Estado de Minas Gerais. Foram isoladas 24 amostras de infecção clínica, 66 de subclínica e 22 de infecção crônica. As amostras de infecção crônica foram isoladas repetidas vezes dos mesmos quartos mamários de nove vacas de um rebanho, no período de 13 meses. A CMI foi realizada em ágar Mueller Hinton, com concentrações entre 0,015 e 128µgml-1 de cada antimicrobiano. As amostras da American Type Culture Collection (ATCC) recomendadas para controle de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mastite bovina; Staphylococcus aureus; Susceptibilidade antimicrobiana; Concentração mínima inibitória; Beta-lactamase. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500003 |
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Registros recuperados: 148 | |
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